![]() The secondary structure fractions calculated from the CD and FT-IR results show excellent agreement.įigure 1. Secondary structure estimation (SSE) was performed for each concentration of antibody using the amide I peak at 1700-1600 cm -1. The same samples were measured by FT-IR spectroscopy, which is an orthogonal method to CD, to verify the plausibility of the calculated secondary structure fractions (Fig. The MRE spectra for each IgG concentration are consistent, and there is no change in the calculated secondary structure in this concentration range (Figs. Concentration calculation and subsequent conversion to MRE were also automatically conducted by Spectra Manager™ 2.5 BeStSel CFR using a sequence of antibodies. The MRE spectra are obtained from raw CD spectra normalized by concentration. The intensity of the raw CD spectra increases linearly with concentration. 1a and 1b show simultaneously measured CD and absorption spectra for various concentrations of IgG. Here, we describe an expanded application of CD spectroscopy for highly concentrated antibodies (undiluted) and report the results of a detailed secondary structure evaluation of antibody drugs using Spectra Manager™ 2.5 BeStSel CFR.įirst, we examined the validity of measuring highly concentrated samples using short-pathlength cells with IgG from rabbit serum. To make BeStSel accessible to biopharma, we developed Spectra Manager™ 2.5 BeStSel CFR as an add-in software for Spectra Manager™, a control and analysis platform for JASCO CD spectrometers, which is compatible with GxP. While many academic researchers use the BeStSel web server, researchers in biopharma companies who need to work in a GxP environment have not been able to benefit from BeStSel. BeStSel has the following features: 1) high estimation accuracy for a wide range of proteins, including β-strand-rich proteins such as antibodies, 2) provides eight types of secondary structure information, 3) capability to predict protein folding following the CATH classification, and 4) open web server. ![]() developed the BeStSel algorithm that can accurately estimate the secondary structure from a CD spectrum by taking into account parallel-antiparallel orientations of β-strands and the twist of antiparallel β-sheets. ![]() Secondary structure estimation offers a route to evaluate protein higher order structure, but historically has not worked well on proteins with high amounts of β-sheet such as antibodies. Fortunately, evaluation of high concentration solutions can be done via short CD path lengths (~10 mm) or using FTIR with ATR. The ability to perform structural characterization of antibodies without dilution is extremely important because intermolecular interactions and changes in the HOS can lead to the formation of aggregates that cause serious immunogenicity. Under these conditions, the antibody molecules exhibit physicochemical properties that differ from those under dilute conditions. Although CD spectroscopy can provide robust structural information for low-concentration protein solutions (< ≈0.1 mg/mL), antibody drugs are formulated at concentrations higher than 10 mg/mL. 1) The most well-known method for HOS characterization is circular dichroism (CD) spectroscopy. Higher-order structure (HOS) is an important antibody drug physicochemical property.
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![]() Platform Web browser, Mobiles, Tablets, PC, SEGA, SNES, Emulator. The Flintstones - The Surprise at Dinosaur Peakĭate added Chicago Time: 01:15 On 28 April 2023 The Flintstones: The Rescue of Dino & Hoppy Enjoy the story and characters, and don't get too frustrated if you fail at certain challenges. Have fun: Remember that the most important thing is to have fun while playing the game. Save often: The game can be challenging, so save your progress often to avoid having to replay large sections of the game. Experiment with different strategies to find what works best for you.ĥ. Experiment with different strategies: There are several ways to approach each level and defeat enemies. ![]() Pay close attention to the environment and use Flik's abilities to avoid these hazards.Ĥ. Pay attention to the environment: The game's levels are filled with hazards such as water, spikes, and other obstacles. Make sure to use these abilities wisely to navigate through the levels and defeat enemies.ģ. Use Flik's abilities wisely: Flik has several abilities, including jumping, crawling, and throwing berries. Explore every nook and cranny of each level to find all the hidden items.Ģ. Collect all the items: The game is filled with hidden items and collectibles that can be used to unlock new levels and gain rewards. Here are a few tips for playing A Bug's Life game:ġ. ![]() Overall, A Bug's Life is considered a classic game of its era and is still enjoyed by fans of the movie and platforming games. Critics praised the game's visuals, music, and voice acting, although some criticized the game's difficulty level. The game received generally positive reviews for its faithful adaptation of the movie's story and characters and its solid gameplay mechanics. The game also features mini-games and side quests that allow players to collect items and earn rewards. The gameplay involves platforming and puzzle-solving elements as Flik navigates various environments and battles enemies. The game follows the story of Flik, an ant who sets out to save his colony from a group of evil grasshoppers led by Hopper. It was developed by Traveller's Tales and published by Disney Interactive Studios for various gaming platforms, including PlayStation, Nintendo 64, Microsoft Windows, and Game Boy Color. What can you tell me about A Bug's Life game?Ī Bug's Life is a video game based on the Disney-Pixar animated film of the same name. Flik's special moves include the following as well: running, jumping, attacking, and picking up things. Utilize Flik's unique abilities to your advantage, such as running, jumping, attacking, picking up things, growing plants, throwing plants, using a grain harvester, butt-bouncing, swinging, and sliding. ![]() You take on the role of Flik, the film's main character, while you play this game. Back in 1998, the video game was available on the Nintendo 64 (N64) gaming platform. Not only that, but craft and upgrade your armor and weapons to give your character the stats and advantages you need in order to take on the perils of the backyard.Play A Bug's Life game online free and unblocked on Brightestgames!Īnd join a dazzling adventure in this classic Disney Pixar game inspired by the film A Bug's Life now online in the video game A Bug's Life. Use the in-game customization systems such as Mutations and Milk Molars to activate the bonuses and perks you want for your character. However, the more you interfere with the creatures in the yard, the higher the chance that they come knocking at your own door, so you better prepare. You can even attract them to different places in the yard by activating the MIX.R devices. Not only that, but with the Shared Worlds feature, you can continue to play in your shared world even if the original host is not on, with all your progression saving!Ĭreatures can be found roaming the yard in a multitude of environments, such as the depths of the pond, the caverns of the termite den, and even the sweltering heat found in the sandbox. You can face the backyard alone or together, online, with up to three friends. How did you wind up so small? Who did this to you? How do you go home? These are all answers you will uncover as you play through the story. Uncover the mysteries while playing through the story! Can you thrive alongside the hordes of giant insects, fighting to survive the perils of the backyard? Explore, build and survive together in this first-person, multiplayer, survival adventure. The world is a vast, beautiful, and dangerous place – especially when you have been shrunk to the size of an ant. ![]() Web free business card word template business card design template free free business card background template blank. Web announce who you are and what you do with these customizable business card templates and designs. Web make a great impression with our free professionally designed business card templates. Blank Business Card Template Free For Word Addictionary Blank Business Card Template Word Fresh Business Card Free Templates Or if you want a different way to design, check out our free business card templates that you can customise online for free.Blank Business Card Word Template. Your business card is now ready to print! So, what do you say? Upload your new business card design now to send it to print.įor more advice or if you’d like a second pair of eyes to look over your template or design, get in touch with our team and we’ll be happy to help. Then, under the ‘Save as type’ drop-down menu, choose PDF.Choose where you want to save this file to.The easiest way to do this is to save your finished file as a PDF: To make sure everything prints properly and the template stays in the right place, you’ll need to save your file in a way that embeds or flattens these elements – which is easier than it sounds! You’ve probably added lots of different elements to your business card design, like text and your logo. If you’re not sure on what colour or font you want or where to put your logo, you’re in luck! We recently conducted a study to find the scientifically perfect business card design. Change the colour, borders and shadows under the Format tab.Draw the shape by clicking and dragging on your document.Choose a shape you’d like to add to your design.Drag and drop your logo so it sits where you want it to on your business card designĪdding a shape to your business card is a great way to differentiate the design from others. ![]() To add an image, like your logo to your design: *Please note that some colours may appear duller after printing. Time for the fun bit! Give your business card some colour or add your logo and create a business card that’s unmistakably yours. You can change your font, size and colour under the Home tab. ![]()
![]() The Triangle Mastercard and the Triangle World Elite Mastercard do not have an annual fee. The billing period covered by each statement can be from 28-33 days. For residents of Quebec, the period between the statement date and the due date for payment is 26 days. The minimum payment is the sum of (a) the greater of: (i) interest and fees shown on your statement + $10 or (ii) 5% of the New Balance, excluding amounts on special payment plans, (b) any balance over your credit limit, (c) any amounts past due not included in (b) above, and (d) the amount of any equal payments plan instalments then due. Some applicants may receive a higher or lower regular annual rate depending on a credit evaluation. Offer subject to change without notice.Īdditional information for residents of Quebec only: The regular annual rate for persons applying for the Triangle Mastercard and the Triangle World Elite Mastercard is 21.99% for cash transactions and related fees and 21.99% for all other charges. Any unpaid portion not received by the due date will no longer form part of the equal payments plan and interest will accrue on that amount from the day after the date of your next statement at the applicable regular annual rate. Each month during an equal payments plan you are required to pay in full by the due date that month’s equal payments plan instalment. There is no administration fee charged for entering into a special payments plan. However, if we do not receive the full minimum due on a statement within 59 days of the date of that statement, or any event of default (other than a payment default) occurs under your Cardmember Agreement, all special payment plans on your account will terminate and (i) you will then be charged interest on the balances outstanding on such plans at the applicable regular annual rate from the day after the date of your next statement, and (ii) the balances outstanding will form part of the balance due on that statement. Interest does not accrue during the period of the plan. Dealers may sell for less.įinancing available is “Equal payments, no interest” for 24 months (unless otherwise stated) and is only available on request, on approved credit and on purchases of $150 (unless otherwise stated) or more (Gift Cards excluded) made with your Triangle credit card at Canadian Tire, Sport Chek, Mark’s, L’Équipeur, Atmosphere, Sports Rousseau, Hockey Experts, L’Entrepôt du Hockey and participating Sports Experts. **Online prices and sale effective dates may differ from those in-store and may vary by region. ±Was price reflects the last national regular price this product was sold for. The tire producer / manufacturer and Canadian Tire uses this fee to pay for the collection, transportation, and processing of used tires.ĬANADIAN TIRE® and the CANADIAN TIRE Triangle Design are registered trade-marks of Canadian Tire Corporation, Limited. To liven up the festive ambiance and enjoy family gatherings, holidays, parties, and fun-filled celebrations.△The tire producer / manufacturer of the tires you are buying, and Canadian Tire is responsible for the recycling fee that is included in your invoice. Try this awesome Christmas gift idea to relive your childhood and add extra fun to kids' daily routines. 【 Christmas Gift Ideas】Stylish gifts for kids and adults. ![]() Loosen the screw under the light to find the ideal angle. Prepare Christmas lights suit every need perfectly with the adjustable heads. 【 180° Adjustable Projector Head】Project the light toward the ceiling, wall, window, floor, or anywhere you wish.Let Santa greet your friends the moment they enter! Say no to messy string lights and turn on your easy-setup projector lights. Its waterproof IP65 structure and IP44 cable endure in snow and rain. Multiple ways to mount or stake into the ground. 【 Versatile Mounting】Ground stake and stand base.For optimal effect, it’s advised to project the light toward surfaces with little obstruction. 【 Brighter and Clearer Patterns】The built-in 5W LED light and upgraded films with vibrant animated images ensure higher brightness and clarity, and the light works well even when it rains or snows.Vibrant and adorable sleigh bells and Santa with his gifts liven up the merry atmosphere at parties and holidays. 【 Animated Patterns Add Holiday Spirits】Three different slides with 6 Christmas and New Year-themed patterns will impress your neighbors and delight partygoers of all ages.Get one projector light for Christmas and the new year, making the festive decorations even merrier and easier! Description Additionally, the projector lights are ideally suited for gift-giving purposes. The lights are optimal for the Christmas party decor of rooms, gardens, garage doors, front yards, and more. Suitable for indoor and outdoor use with a robust and IP65 waterproof construction. Create a warm and cozy feel throughout your house ![]() When you think of Halloween, you think of pumpkins right? Make sure everybody knows Halloween is coming up with a Pumpkin Halloween virtual call background, or add some creepy skies and bats to the mix. We bring the best scenes from Halloween movies to your Microsoft Teams, Zoom or Webex virtual backgrounds! Scary Movies Virtual Backgrounds Harry Potter and the Sorcerer’s Stone Corpse Bride IT Friday the 13th Texas Chainsaw Massacre The Haunted Mansion The Conjuring 2 The Shining The Nightmare Before Christmas Fun Halloween Virtual Backgrounds If you’re squeamish, act with caution: these backgrounds may give your work colleagues a fright, but they may catch you first! We have the best virtual backgrounds from scary movies below, including the Harry Potter Halloween scene background and the iconic characters from Corpse Bride. With the spooky season of Halloween creeping up on us, we want you to be readily armed with some awesome virtual Halloween backgrounds for Microsoft Teams and Zoom. If you are looking to jazz up your video call backgrounds (some call them wallpapers), these make a great conversation starter for all of your virtual meetings! are not copyrighted and are intended for public use. If you are looking for additional fun Microsoft Teams or Zoom virtual backgrounds, check out our blog on ‘Fun Microsoft Teams Backgrounds’ here.Please note that all Teams background images shared by Regroove Solutions Inc. ![]() Regroove Tipsicle – Break the ice in Microsoft Teams and Zoom Meetings with Snapchat Snap Camera Looking for more ways to break the ice in your Microsoft Teams and Zoom meetings? Check out Sean’s video on Snapchat Snap Camera below! (Optional) Check Require users to always use virtual background then Save to confirm the change.(Optional) Click Manage virtual background to upload default background images available for users. Users must have client/app version 5.1.1 or higher to see the background images you upload. ![]() This grants access to upload virtual backgrounds in addition to the backgrounds currently uploaded. Check ‘ Allow users to upload custom backgrounds’ then ‘ Save’ to confirm the change.In the Meeting tab, navigate to the ‘ Virtual Background’ option (under the In Meeting (Advanced) section) and verify that the setting is enabled.In the navigation menu, click ‘ Account Management’ then ‘ Account Settings’.Regroove alumni Kyle has put together a video on how to add Custom Virtual Backgrounds to Microsoft Teams. You can check that out here: If you are looking to change your background during a Microsoft Teams meeting, go to your meeting controls and select ‘ More actions’ > ‘ Apply background effects’. Select Preview to see how your chosen background looks before you apply it, and then select Apply.Your new background will stay and persist in all of your Microsoft Teams meetings and calls until you change it.To do this, select ‘ Add new’ and select one to upload from your computer. ![]()
![]() A widget that could show a specific picture. The second most critical part of creating an aesthetic screen is the photo widget. ![]() If it feels satisfying enough, repeat the steps above to create icons for all the apps to put on your aesthetic screen. You can rearrange it just like any other app. ![]() Go to your iPhone home screen to check the new app icon in action. So, open the ‘Shortcuts’ app on your iPhone from the App Library. To create custom app icons, we’ll be using the shortcuts app. You can proceed with creating shortcut icons for each app that you want to customize. Once you’ve saved the pictures you want to use as app icons on your iPhone. Neon aesthetic icons with black background Feel free to use them for your own aesthetic screens. We created 512×512 pixel sized icons and put a black background behind so they appear aesthetic enough when used as icons. Our aesthetic for this guide is neon, so we’ll be using some neon pictures to match the aesthetic.īelow are some of the neon icons we found on Icons8. To get started we need to download and save a bunch of aesthetic pictures that we could use as custom icons for the apps. Creating custom app icons that matches your home screen aesthetic can be a challenging task but it’s actually easier done than said. Wallpaper by Radu Spătaru ( Download link) Probably, any dark themed wallpaper (like the one below) will do. Since we’re creating a neon aesthetic home screen, we’ll choose and select a wallpaper that complements neon aesthetics. Once we have a clean screen, it’s time to set a wallpaper that is aesthetic what you’re trying to build. Set a Wallpaper that Meets your Aesthetic Requirements Safely remove all app icons so you have a clean home screen with no app icons other than the ones on the dock. Then, tap the ‘- minus’ icon on the top-left of each app icon. Tap and hold on any app until the screen goes jiggly. Now, empty the only home screen page you have by removing all app icons from it (widgets too, if any). Remove All App Icons to Get a Clean Home Screen Tap ‘Done’ on the top-right corner or just go to home screen by swiping up from the bottom or pressing the Touch ID button to save your changes. Keep only one home screen page and hide everything else Now, untick all your home screen pages and keep only one page. Then, tap the dots at the bottom of the screen. To hide home screen pages on iPhone, tap and hold on any empty space on your home screen to enter the jiggly mode. You’ll still be able to access your apps from the App Library on your iPhone. Do that by hiding all current pages on your device. Clear your home screenīefore we get started, you need to de-clutter your iPhone home screen. We’ll be particularly creating a Neon aesthetic screen complete with related widgets and app icons.īut before we get started, for inspiration, we recommend you give yourself a glimpse of what aesthetic screens other users have created on our iOS 14 aesthetic ideas page. We’ll detail everything about creating an aesthetic home screen in this guide. People are creating home screens with photo widgets and custom app icons in iOS 14 in a very controlled style that the overall over home screen appears to be aesthetic of one particular style. Ever since the iOS 14 update began rolling out, Aesthetic home screens have been are all the craze among iPhone users. |
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